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Mouse brain hemisphere labeled with 5 µg of goat anti-GFP (Encor Biotechnology, CAT# GPCA-GFP). Imaged with SmartSPIM at 1.8 µm lateral pixel size and 4 µm z-step.
a) eGFP acquired at 488-nm, 400-µm maximum intensity projection. Scale bar 250 µm.
b) anti-GFP acquired at 561-nm, 400-µm maximum intensity projection. Scale bar 250 µm.
c) Merged image of two MIPs, showing colocalization of endogenous GFP and anti-GFP labeling.
Mouse brain hemisphere with ChAT+ neurons in both the brainstem and basal forebrain with dense labeling of Neuropeptide Y throughout the hypothalamus.
Tissue immunolabeled anti-NPY (cyan) and anti-ChAT (magenta). Imaged with SmartSPIM at 4 µm Z-step and 1.8 µm XY pixel size.
Tissue courtesy of Dr. Hongwei Dong, Center for Integrative Connectomics (CIC) lab, USC.
Retrogradely labeled motor cortical neurons projecting to striatum via rabies virus expressing eGFP. Imaged with SmartSPIM.
Sample courtesy of Dr. Byungkook Lim, UCSD.
Nuclear stain (SYTO16, in cyan) and vasculature stain (DyLight 649-conjugated tomato lectin, pink). Imaged with SmartSPIM.
Tissue courtesy of Translational Bioimaging Group, Barrow Neurological Institute.
Mouse duodenum segment, perfused with lectin (red) to label vasculature and immunolabeled with anti-Olfm4 stem cell marker (cyan). Imaged with SmartSPIM at 2 µm Z-step and 1.8 µm XY pixel size.
Sample courtesy of Dr. Suhail Chaudhry, Ferrara Bone Marrow Transplantation Lab, Icahn School of Medicine.
MIP (200µm) image showing TH labeling of Ventral Tegmental Area (VTA), Substantia Nigra (SN), Retrorubral Field (RF), and Locus Coeruleus (LC), highly populated with dopaminergic and noradrenergic neurons.
Imaged with SmartSPIM at 1.8 x 1.8 x 4 µm voxel size.
tdTomato labeling of a mouse spinal cord cell-type, imaged with SmartSPIM at 1.8 x 1.8 x 4 µm voxel size.
Sample courtesy of Dr. Helen Lai, UT Southwestern.
Tissue treated with lectin dye, labeling blood vessels with high specificity and signal-noise ratio.
Imaged with SmartSPIM 15X objective, 1.5 x 1.5 x 1.8 mm region acquired at 1 µm Z-step.
Immunolabeled GFAP (cyan) and Beta-amyloid plaques (magenta) from the brain of a 5xFAD Alzheimer’s disease model mouse.
Imaged with SmartSPIM at 1.8 x 1.8 x 4 µm voxel size. Sample courtesy of Drs. David Elliott and Jonathan Epp, University of Calgary.
a) Radially-positioned hair cells within the spiral cavity of the cochlea surrounded by bone and nerve tissue.
b) Cut end of a nerve bundle.
MIP (200 µm) imaged with SmartSPIM at 4 µm z-step and 1.8 µm XY pixel size. Tissue courtesy of Dr. Rick A. Rogers, Harvard School of Public Health.