Image Gallery

Figure 1. Thy1-YFP animal. Tissue was fixed with SHIELD, cleared in SmartClear 2 Pro, and imaged with SmartSPIM

a) Each image is a 50 µm max z-projection. The frames are separated by 300 µm. The raw data was collected at 5 µm axial spacing, with a 1.85 µm lateral pixel size. The 10.2mm x 16.7mm x 5.6mm volume was acquired in 15 minutes.

b) The slice in a) outlined in red is shown. Scale bar = 2 mm. Gamma adjusted to 0.4.

Figure 2. Homogenous and high axial performance throughout. Thy1-YFP animal.

a) Shown is the outlined area from Figure 1b.

b) The stack was virtually resliced along Y (blue outline in a)) to show the axial resolution. Shown is a 50 µm max y-projection.

c) Expanded region from a) outlined in red.

d) Expanded region from b) outlined in red.

Scale bar = 500 µm for a) and b). Scale bar = 100 µm for c) and d).

Figure 3. Staining and imaging. A hemisphere was fixed with SHIELD, cleared in the SmartClear, nuclear labelled (To-Pro-3) with SmartLabel, and imaged with the SmartSPIM.

a) Each image is a single slice from the z-stack. The frames are separated by 300 µm. The raw data was collected at 4 µm axial spacing, with a 1.85 µm lateral pixel size. The 10.0mm x 18.8mm x 7.6mm volume was acquired in 25 minutes. b) The slice in a) outlined in red is shown. Scale bar =  2 mm. Gamma adjusted to 0.4.

Figure 4. Homogenous and high axial performance throughout. To-Pro-3 labelled animal.

a) Shown is the outlined area from Figure 3b for a single XY slice.

b) The stack was virtually resliced along Y (blue outline in a)) to show the axial resolution. Shown is a single XZ slice.

c) Expanded region from a) outlined in red.

d) Expanded region from b) outlined in red.

Scale bar = 500 µm for a) and b). Scale bar = 100 µm for c) and d).

Figure 5. Retrogradely labeled motor cortical neurons projecting to striatum using rabies virus expressing eGFP.

a) Max projection of 2mm. Scale bar = 200 µm b) Zoomed in region from a). Scale bar = 50 µm. Tissue was cleared and imaged with the SmartSPIM in the laboratory of Prof. Byungkook Lim at UCSD.