Mouse brain hemisphere labeled with 5 µg of goat anti-GFP (Encor Biotechnology, CAT# GPCA-GFP). Imaged at 1.8 µm lateral pixel size and 4 µm z-step.
a) eGFP acquired at 488-nm, 400-µm maximum intensity projection. Scale bar 250 µm.
b) anti-GFP acquired at 561-nm, 400-µm maximum intensity projection. Scale bar 250 µm.
c) Merged image of two MIPs, showing colocalization of endogenous GFP and anti-GFP labeling.
Mouse brain hemisphere with ChAT+ neurons in both the brainstem and basal forebrain with dense labeling of Neuropeptide Y throughout the hypothalamus.
Tissue immunolabeled anti-NPY (cyan) and anti-ChAT (magenta). Imaged at 4 µm Z-step and 1.8 µm XY pixel size.
Tissue courtesy of Hongwei Dong, Center for Integrative Connectomics (CIC) lab, USC.
Nuclear stain (SYTO16, in cyan) and vasculature stain (DyLight 649-conjugated tomato lectin, pink).
Tissue courtesy of Translational Bioimaging Group, Barrow Neurological Institute.
Mouse duodenum segment, perfused with lectin (red) to label vasculature and immunolabeled with anti-Olfm4 stem cell marker (cyan). Imaged at 2 µm Z-step and 1.8 µm XY pixel size.
MIP (200µm) image showing TH labeling of Ventral Tegmental Area (VTA), Substantia Nigra (SN), Retrorubral Field (RF), and Locus Coeruleus (LC), highly populated with dopaminergic and noradrenergic neurons.
Imaged at 1.8 x 1.8 x 4 µm voxel size.
Tissue treated with lectin dye, labeling blood vessels with high specificity and signal-noise ratio.
15X objective, 1.5 x 1.5 x 1.8 mm region acquired at 1 µm Z-step.