Image Gallery

Figure 1. Mouse brain hemisphere processed using the entire LifeCanvas pipeline: SHIELD-fixed, delipidated in SmartClear II Pro, labeled with SmartLabel with only 5 µg of Goat anti-GFP (Encor Biotechnology, CAT# GPCA-GFP), and imaged with SmartSPIM at 1.8 µm lateral pixel size and 4 µm z-step.

a) eGFP acquired at 488-nm laser line, 400-µm maximum intensity projection. Scale bar 250 µm.

b) anti-GFP acquired at 561-nm laser line, 400-µm maximum intensity projection. Scale bar 250 µm.

c) Merged image of the two MIPs, showing colocalization of endogenous GFP and anti-GFP labeling.

Figure 2. Mouse cochlea. SmartSPIM imaging of mouse cochlea autofluorescence.

a) Radially-positioned hair cells are visible within the spiral cavity of the cochlea, which is surrounded by bone and nerve tissue.

b) The cut end of a nerve bundle.

Max projection of 200 µm, imaged at 4 µm z-step and 1.8 µm pixel size. Tissue courtesy of the Rogers Lab at Harvard School of Public Health.

Figure 3. Retrogradely labeled motor cortical neurons projecting to striatum using rabies virus expressing eGFP.

a) Max projection of 2 mm. Scale bar = 200 µm.

b) Zoomed in region from a). Scale bar = 50 µm. Tissue was cleared and imaged with SmartSPIM in the laboratory of Prof. Byungkook Lim at UCSD.

Figure 4. Thy1-YFP animal. Tissue was fixed with SHIELD, cleared in SmartClear II Pro, and imaged with SmartSPIM

a) Each image is a 50 µm max z-projection. The frames are separated by 300 µm. The raw data was collected at 5 µm axial spacing, with a 1.85 µm lateral pixel size. The 10.2 mm x 16.7 mm x 5.6 mm volume was acquired in 15 minutes.

b) The slice in a) outlined in red is shown. Scale bar = 2 mm. Gamma adjusted to 0.4.

Figure 5. Thy1-YFP animal. Homogenous and high axial performance throughout. 

a) Shown is the outlined area from Figure 3b.

b) The stack was virtually resliced along Y (blue outline in a)) to show the axial resolution. Shown is a 50 µm max y-projection.

c) Expanded region from a) outlined in red.

d) Expanded region from b) outlined in red.

Scale bar = 500 µm for a) and b). Scale bar = 100 µm for c) and d).

Figure 6. Staining and imaging. A hemisphere was fixed with SHIELD, cleared in SmartClear, nuclear labeled (To-PRO-3) with SmartLabel, and imaged with SmartSPIM.

a) Each image is a single slice from the z-stack. The frames are separated by 300 µm. The raw data was collected at 4 µm axial spacing, with a 1.85 µm lateral pixel size. The 10.0 mm x 18.8 mm x 7.6 mm volume was acquired in 25 minutes.

b) The slice in a) outlined in red is shown. Scale bar =  2 mm. Gamma adjusted to 0.4.

Figure 7. To-PRO-3 labeled animal. Homogenous and high axial performance throughout. 

a) Shown is the outlined area from Figure 6b for a single XY slice.

b) The stack was virtually resliced along Y (blue outline in a)) to show the axial resolution. Shown is a single XZ slice.

c) Expanded region from a) outlined in red.

d) Expanded region from b) outlined in red.

Scale bar = 500 µm for a) and b). Scale bar = 100 µm for c) and d).