Image Gallery

Mouse brain hemisphere processed using the entire LifeCanvas pipeline: SHIELD-fixed, delipidated in SmartClear II Pro, labeled with SmartLabel with only 5 µg of Goat anti-GFP (Encor Biotechnology, CAT# GPCA-GFP), and imaged with SmartSPIM at 1.8 µm lateral pixel size and 4 µm z-step.

a) eGFP acquired at 488-nm laser line, 400-µm maximum intensity projection. Scale bar 250 µm.

b) anti-GFP acquired at 561-nm laser line, 400-µm maximum intensity projection. Scale bar 250 µm.

c) Merged image of the two MIPs, showing colocalization of endogenous GFP and anti-GFP labeling.

Mouse brain hemisphere processed using the entire LifeCanvas pipeline and labeled with Mouse anti-MBP (Encor Biotechnology, CAT# MCA-7G7). Imaged at 3.6x (pixel size 1.8 x 1.8 x 4 µm), 40 µm max projection.

Retrogradely labeled motor cortical neurons projecting to striatum using rabies virus expressing eGFP.

a) Max projection of 2 mm. Scale bar = 200 µm.

b) Zoomed in region from a). Scale bar = 50 µm. Tissue was cleared and imaged with SmartSPIM in the laboratory of Prof. Byungkook Lim at UCSD.

A mouse brain hemisphere was fixed with SHIELD, cleared in SmartClear, nuclear labeled (To-PRO-3) with SmartLabel, and imaged with SmartSPIM.

Panel 1:

a) Each image is a single slice from the z-stack. The frames are separated by 300 µm. The raw data was collected at 4 µm axial spacing, with a 1.8 µm lateral pixel size. The 10.0 mm x 18.8 mm x 7.6 mm volume was acquired in 25 minutes.

b) The slice in a) outlined in red is shown. Scale bar =  2 mm. Gamma adjusted to 0.4.

Panel 2: 

Homogenous and high axial performance throughout. 

a) Shown is the outlined area from “Mouse brain hemisphere with nuclear label (1)” for a single XY slice.

b) The stack was virtually resliced along Y (blue outline in a)) to show the axial resolution. Shown is a single XZ slice.

c) Expanded region from a) outlined in red.

d) Expanded region from b) outlined in red.

Scale bar = 500 µm for a) and b). Scale bar = 100 µm for c) and d).

These 200-µm MIP images show specific TH labeling of the Ventral Tegmental Area (VTA), Substantia Nigra (SN), Retrorubral Field (RF), and Locus Coeruleus (LC) regions, which are highly populated with dopaminergic and noradrenergic neurons. 

This sample was processed intact through the LifeCanvas pipeline: SHIELD-fixed, delipidated in SmartClear II Pro, immunolabeled with SmartLabel, and imaged with SmartSPIM at 1.8 x 1.8 x 4 µm voxel size.

SmartSPIM imaging of mouse cochlea autofluorescence.

a) Radially-positioned hair cells are visible within the spiral cavity of the cochlea, which is surrounded by bone and nerve tissue.

b) The cut end of a nerve bundle.

Max projection of 200 µm, imaged at 4 µm z-step and 1.8 µm pixel size. Tissue courtesy of the Rogers Lab at Harvard School of Public Health.

Tissue was fixed with SHIELD, cleared in SmartClear II Pro, and imaged with SmartSPIM

Panel 1:

a) Each image is a 50 µm max z-projection. The frames are separated by 300 µm. The raw data was collected at 5 µm axial spacing, with a 1.85 µm lateral pixel size. The 10.2 mm x 16.7 mm x 5.6 mm volume was acquired in 15 minutes.

b) The slice in a) outlined in red is shown. Scale bar = 2 mm. Gamma adjusted to 0.4.

Panel 2: 

Homogenous and high axial performance throughout. 

a) Shown is the outlined area from “Thy1-YFP mouse brain hemisphere (1)”.

b) The stack was virtually resliced along Y (blue outline in a)) to show the axial resolution. Shown is a 50 µm max y-projection.

c) Expanded region from a) outlined in red.

d) Expanded region from b) outlined in red.

Scale bar = 500 µm for a) and b). Scale bar = 100 µm for c) and d).