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Endogenous Thy1-driven YFP expression visualized throughout an entire adult mouse brain

Mouse duodenum perfused with lectin and immunolabeled with anti-Olfm4

tdTomato labeling of a mouse spinal cord cell-type

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Mouse brain hemisphere labeled with 5 µg of Goat anti-GFP (Encor Biotechnology, CAT# GPCA-GFP). a) eGFP acquired at 488-nm laser line, 400-µm maximum intensity projection. Scale bar 250 µm. b) anti-GFP acquired at 561-nm laser line, 400-µm maximum intensity projection. Scale bar 250 µm. c) Merged image of two MIPs, showing colocalization of endogenous GFP and anti-GFP labeling. Imaged at 1.8 µm lateral pixel size and 4 µm z-step.
Mouse brain hemisphere displaying ChAT-positive neurons in both the brainstem and basal forebrain and dense labeling of Neuropeptide Y throughout the hypothalamus. Tissue immunolabeled anti-NPY (cyan) and anti-ChAT (magenta). Tissue courtesy of Hongwei Dong, Bin Zhang, and Neda Khanjani and the Center for Integrative Connectomics (CIC) lab.
200-µm MIP images show specific TH labeling of Ventral Tegmental Area (VTA), Substantia Nigra (SN), Retrorubral Field (RF), and Locus Coeruleus (LC) regions, which are highly populated with dopaminergic and noradrenergic neurons. Imaged at 1.8 x 1.8 x 4 µm voxel size.

Mouse brain labeled with Encor Goat anti-GFP

Mouse right hemisphere dual labeled with anti-Neuropeptide Y and anti-ChAT

Tyrosine Hydroxylase staining in mouse midbrain

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