3D Phenotyping with the SmartSPIM Light Sheet Microscope

After tissue clearing and (optional) labeling, intact tissue needs to be index-matched with EasyIndex refractive index-matching solution. Afterwards, tissues will appear fully transparent and ready for imaging using the SmartSPIM light sheet microscope. To complete your workflow, LifeCanvas Technologies offers SmartAnalytics – a powerful machine learning analysis tool, specially designed for large 3D datasets. This… Continue reading 3D Phenotyping with the SmartSPIM Light Sheet Microscope

How LifeCanvas tissue clearing preserves maximum fluorescent protein signal and tissue integrity

We discuss the second part of your tissue processing journey: active tissue clearing using SmartClear. SmartClear active clearing is rapid, uniform, and nondestructive, ensuring maximum sample integrity.

Superior tissue preservation with SHIELD

Retrogradely labeled motor cortical neurons projecting to striatum using rabies virus expressing eGFP. 10x, Sample courtesy of Prof. Byungkook Lim's lab at UCSD.

In this blog, we will provide key insights in the first step of this journey: tissue preservation using SHIELD. SHIELD is a tissue-gel hybridization method using polyfunctional, flexible epoxides to preserve tissue architecture, endogenous fluorescence, protein antigenicity, and nucleic acids.

Organoids in 3D: Understanding human brain development & disease

Emerging organoid technologies have been used to model human organ development and diseases “in a dish”. Organoids are derived either from induced pluripotent or organ-restricted adult stem cells. These cells are self-renewing, self-organizing and able to grow into 3D structures resembling the architecture and physiology of the original biological specimens.

How 3D tissue clearing benefits COVID-19 research

Over 190 million people have now been infected with the novel coronavirus SARS-CoV-2 in the ongoing coronavirus disease (COVID-19) pandemic with a total number of deaths passing 4.1 million worldwide. Multiple organs are affected by COVID-19, however, the lungs are the main site of disease.

Recent publications leverage 3D histology to provide new insights into neuronal circuitry

3D neuronal projections in a whole mouse brain (Luchsinger et al., Nat Commun. 2021, Supplementary Movie 1). Licensed under CC BY 4.0.

The goal of histology has long been to interrogate complex structures in whole tissue samples. Over centuries, slicing of samples for traditional two-dimensional histology has been indispensable in understanding the structure and morphology of multiple tissue types. However, this approach is limited to yielding images in thin tissue sections, is time-consuming and is error-prone due to tissue distortion and information loss.