Sample preservation with SHIELD for tissue clearing and 3D histology

Recent advances in the tissue clearing field have unlocked the visualization of biology throughout whole organs. Tissue processing methods that render samples optically transparent can be combined with methods that enable the tissue-wide penetration of molecular probes such as antibodies. However, many of these techniques – which can include dehydration, delipidation, high temperature exposure, and… Continue reading Sample preservation with SHIELD for tissue clearing and 3D histology

Talking transparency: The history and physics of tissue clearing

There is a growing trend among biomedical researchers to interrogate biological structures and biomolecular information in three-dimensional volumes rather than in thin slices. This is particularly the case for neuroscientists looking to spatially map the interactions between the hundreds of unique cell types in the mammalian brain. The ability to perform three-dimensional imaging and phenotyping… Continue reading Talking transparency: The history and physics of tissue clearing