Vasculature damage is a hallmark of many pathological conditions and is of particular interest to researchers studying stroke. However, vascular networks are difficult to study in fine detail at a regional or whole- organ level. By utilizing a dye-based approach that is compatible with LifeCanvas’s clearing device, researchers can achieve rapid high-resolution imaging of organ-scale tissues.
Intravenously-delivered fluorescent dye labeling of an extensive network of mouse brain blood vessels, imaged at high resolution with fine detail.
Image courtesy of Profs. David Kleinfeld and Byungkook Lim, UCSD.
This mouse whole brain was stained using SmartLabel with a tomato lectin dye, which labels blood vessels with high specificity and high SNR. SmartLabel is the only active labeling system able to stain structures deep in the core of the brain with incredible uniformity in only 24 hours. This whole adult mouse brain was stained with only 40 µL of the lectin dye. This is achieved by combining two powerful technologies, stochastic electrotransport and SWITCH, which drives the antibodies or dye molecules deep into the core of the tissue before allowing binding.
The 1.5 x 1.5 x 1.8 mm region shown in the video was acquired with a 15x objective with SmartSPIM at 1 µm z-step in less than 5 minutes. Lateral pixel size is 0.412 µm.
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