SmartSPIM is an advanced light sheet microscope capable of imaging large tissue samples with high speed and resolution. The system was designed from the ground up, with no limitation based on commercially available microscope bodies. The unique illumination arm of the SmartSPIM creates a flat-top illumination profile laterally, and a focused beam axially. To achieve homogeneous axial point spread functions (PSF) across the entire FOV, the illumination optics scan the beam axially while synchronized to the camera’s detection.
Dynamically axially swept light sheet
NA = 0.2 broadband chromatically corrected
2 cm x 3 cm x 1.2 cm
Infinity corrected (180 mm EFL tube lens). Dual detection cameras possible with custom beam splitting optics.
3.6x = 1.8 µm/pix; 10x = 0.64 µm/pix
3.6x, NA 0.2, PSFz < 4.5µm ; 10x*, NA 0.6, PSFz < 2.5 µm
3.6x = 3700 µm ; 10x* = 1300 µm
8-12 FPS during z-stack acquisition
2048 x 2048 sCMOS with rolling shutter
– synchronized to light sheet for confocal detection
Up to 6 in a single combiner.
Wavelengths from 488 – 785 nm
* Olympus 10x objective (XLPLN10XSVMP)
SmartSPIM image of fluorescent protein expression in a subset of neurons. High-resolution axial spacing (4µm) enables seamless display of original XY-dimensional data in orthogonal directions (XZ & YZ), providing for accurate identification of each cell body