SmartSPIM

Light sheet imaging system optimized for resolution, speed, and flexibility for large samples

SmartSPIM is an advanced light sheet microscope capable of imaging large tissue samples with high speed and resolution. The system was designed from the ground up, with no limitation based on commercially available microscope bodies. The unique illumination arm of the SmartSPIM creates a flat-top illumination profile laterally, and a focused beam axially. To achieve homogeneous axial point spread functions (PSF) across the entire FOV, the illumination optics scan the beam axially while synchronized to the camera’s detection.

microscope

Features & Highlights

 

  • Capable of imaging a wide range of sample sizes

 

  • High axial resolution with many different objectives

 

  • Uniform axial resolution across the entire sample

 

  • High speed volumetric imaging

 

  • Ability to use custom and commercially available objectives

 

  • Custom illumination and detection optics optimized for all current large format objectives and camera

 

  • Sequential and simultaneous multi-color imaging capabilities

 

  • Remote focusing relay to provide focus compensation for RI mismatches and chromatic focal plan
 
Please email imaging@lifecanvastech.com to learn more.
Adult mouse hemisphere imaging acquired with SmartSPIM (8X downsampled from original movie)
Entire hemisphere was stained in 1 day with SmartLabel using SYTO 16 green fluorescent nucleic acid stain and imaged using the SmartSPIM with a custom LifeCanvas 3.5x objective. Acquisition time was less than 1 hour. The high and uniform axial resolution allows for clear delineation of cell bodies in XY and importantly in Z.

Specifications

LIGHT SHEET FORMATION

Dynamically axially swept light sheet

ILLUMINATION OPTICS 

NA = 0.2 broadband chromatically corrected

SPECIMEN SIZE (MAX) 

2 cm x 3 cm x 1.2 cm

DETECTION OPTICS 

Infinity corrected (180 mm EFL tube lens). Dual detection cameras possible with  custom beam splitting optics.

LATERAL SAMPLING 

3.6x = 1.8 µm/pix; 10x = 0.64 µm/pix

AXIAL RESOLUTION  

3.6x, NA 0.2, PSFz <  4.5µm ; 10x*, NA 0.6, PSFz <   2.5 µm

FIELD OF VIEW (FOV) 

3.6x = 3700 µm ; 10x* = 1300 µm

IMAGING SPEED 

8-12 FPS during z-stack acquisition

CAMERA 

2048 x 2048 sCMOS with rolling shutter

– synchronized to light sheet for confocal detection

LASER LINES 

Up to 6 in a single combiner.

Wavelengths from 488 – 785 nm

* Olympus 10x objective (XLPLN10XSVMP)

SmartSPIM image of fluorescent protein expression in a subset of neurons. High-resolution axial spacing (4µm) enables seamless display of original XY-dimensional data in orthogonal directions (XZ & YZ), providing for accurate identification of each cell body